In-vitro models for wound healing, skin biology research and drug discovery
Monolayer (2-D) primary skin cell culture (fibroblasts, keratinocytes, melanocytes)
3-D primary skin cell culture (fibroblast-populated collagen lattices, cultured full thickness epidermis)
Cell co-culture (for direct cell-cell interaction studies)
Double-chamber cell co-culture (for paracrine interaction studies)
Organotypic co-culture model (cultured skin equivalents)
Organ culture (explant techniques)
Applications: These models can be used to study different facets of skin cell biology such as proliferation, apoptosis, migration and differentiation as well as epithelial-mesenchymal interactions, transitions and other cell-cell interactions.
In-vitro angiogenesis models (using endothelial cell culture and smooth muscle cell culture)
Monolayer (2-D) endothelial cell culture
3-D primary cell culture (endothelial cell-populated collagen lattices or Matrigel assay to study endothelial cell differentiation)
Monolayer co-culture (for direct cell-cell interaction studies)
Double-chamber co-culture (with smooth muscle cells or other cells for paracrine interaction studies)
Vascularised organotypic co-culture model (to study endothelial cell-fibroblast-keratinocyte interactions)
Organ culture (Nicosia’s technique)
Applications: These models can be used to study endothelial cell and angiogenetic biology, specifically proliferation, apoptosis, migration and differentiation as well as intercellular interactions and transitions.