Technical

 

Can I freeze CellResearch’s cell strains?

The cells need to be cryopreserved, and likewise thawed, using specific protocols. Simple freezing in a 4 degree Celsius fridge is not advisable as this may lead to a degradation of the cells and their proliferating potential.

 

What techniques are you using to isolate the cell strains?

Explant Technique is used in the isolation of the cell strains.

 

Are CellResearch’s cells strains derived from a single individual or are they pooled from several donors?

CellResearch’s cell strains are derived from single donors and are not pooled.

 

Which anatomical regions are the cell strains derived from?

We have cell strains from various anatomical regions such as earlobe, groin, wrist, leg, etc.  Please consult our sales specialists.

 

Are the Keloid fibroblasts considered cancerous?

The Keloid fibroblasts are not cancerous, but may be considered a benign tumor with autonomous characteristicsas rapid proliferation, local invasiveness and fast migration. We have found that these cells have down-regulation of apoptotic genes and up-regulation of anti-apoptotic genes in addition to other autonomous characteristics.

 

Which cells produce more collagen?

Keloid fibroblasts produce approximately 20 times more collagen than normal dermal fibroblasts while aging fibroblasts produce less collagen than young skin fibroblasts.

 

Which cells are more elastic?

Keloid and Hypertrophic Scar cells are less elastic than normal skin cells.

 

Are the cells tested against potential infectious hazards?

The cells are analyzed for HIV, HBV and HCV. However, please note that CellResearch’s cell cultures are of human origin and while every effort has been taken to test the cells, no diagnostics tests can ensure the total absence of infectious agents. All cells of human origin should be treated as potential pathogens.   

 

General

 

Why should I use CellResearch’s cell strains?

CellResearch’s cell strains are obtained from single donors and are not pooled. We have strains derived from multiple locations of the body. Beside normal human skin, we also have cell strains derived from Keloid and Hypertrophic Scars. For comparative research, fibroblasts isolated from normal skin in the transition zone immediately adjacent to the Keloid or Hypertrophic Scar is also available.

 

How are the cells supplied?

As proliferating cells in culture flasks unless specified. Cryopreserved vials are also available upon request.

 

If your questions have not yet been answered, please contact our Technical Specialists. They will be most happy to clarify your queries.

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